“Raptus Sabinae?” complemented: molecular genetic studies on a female calvarium of the Bandkeramik settlement of Rovantsi in Volhynia (UA)

Janine Mazanec; Susanne Hummel; Thomas Saile

doi:10.23858/SA/72.2020.2.1844

Published: 2020-12-16

Vol. 72 No. 2 (2020)

“Raptus Sabinae?” complemented: molecular genetic studies on a female calvarium of the Bandkeramik settlement of Rovantsi in Volhynia (UA)

Janine Mazanec , Susanne Hummel , Thomas Saile

Sprawozdania Archeologiczne

Section: Field Survey and Materials

https://doi.org/10.23858/SA/72.2020.2.1844

Abstract

A fragmented human cranial calotte was discovered in a Bandkeramik (LBK) settlement context at Rovantsi in Volhynia (UA). The female calvarium of a mature woman with an age of about 45-50 years was uncovered in the deepest part of a settlement pit. It can be dated to round about 5,250 BC. PCR-based molecular genetic analyses were successfully performed on these extremely rare skeletal remains from the Early Neolithic of Ukraine. The female family line can be assigned to haplogroup T2, in which it represents the lineage T2c1d+152. The woman was lactose intolerant, like most LBK individuals. Her hair colour was brown, and her eye colour was found to be hazel.

Keywords:

ancient DNA (aDNA), human mitochondrial DNA haplogroup, DNA profiling, polymerase chain reaction (PCR), Early Neolithic, Bandkeramik (LBK), Ukraine

Most read articles by the same author(s)

Jacek Forysiak, Sławomir Kadrow, Agnieszka Noryśkiewicz, Daniel Okupny, Thomas Saile, Juliusz Twardy, Izabela Zawiska, The environmental context of Early Neolithic cultural transformation in the Targowisko settlement region (Southern Poland) , Sprawozdania Archeologiczne: Vol. 73 No. 1 (2021): Sprawozdania Archeologiczne
Sławomir Kadrow, Martin Posselt, Thomas Saile, Marcin Wąs, Joanna Abramów, Adam Golański, Culture transformation in the Targowisko microregion. Trends of changes among Danubian farmers , Sprawozdania Archeologiczne: Vol. 73 No. 1 (2021): Sprawozdania Archeologiczne
Dmytro Kiosak, Maciej Debiec, Thomas Saile, Stanislav Terna, Lithic industry of two Linearbandkeramik sites in Moldova (Nicolaevca V and Ţâra II) , Sprawozdania Archeologiczne: Vol. 73 No. 1 (2021): Sprawozdania Archeologiczne
Andrzej Pelisiak, Thomas Saile, Maciej Dębiec, A contribution to research on the knapped lithic assemblage from the Late Neolithic site of Altheim in Lower Bavaria , Sprawozdania Archeologiczne: Vol. 76 No. 1 (2024): Sprawozdania Archeologiczne
Andrij Bardec’kyj , Maciej Dębiec, Thomas Saile, Zwei runde Spondylusklappen aus der bandkeramischen Siedlung von Rovancì in Wolhynien. Ein Beitrag zu Tausch und Status im frühen Neolithikum , Sprawozdania Archeologiczne: Vol. 68 (2016)
Maciej Dębiec, Martin Posselt, Thomas Saile, Tyrawa Solna. Salz, Siedlungen und eine Magnetometerprospektion an der Tyrawka in den Salzbergen der Beskiden , Sprawozdania Archeologiczne: Vol. 67 (2015)
Andrij B. Bardec’kyj, Maciej Dębiec, Thomas Saile, Eine bandkeramische Bestattung aus Baїv bei Luzk in Wolhynien = Pochówek kultury ceramiki wstęgowej rytej z miejscowości Baїv koło Łucka , Sprawozdania Archeologiczne: Vol. 65 (2013)

Download files

PDF

Citation rules

Mazanec, J., Hummel, S., & Saile, T. (2020). “Raptus Sabinae?” complemented: molecular genetic studies on a female calvarium of the Bandkeramik settlement of Rovantsi in Volhynia (UA). Sprawozdania Archeologiczne, 72(2). https://doi.org/10.23858/SA/72.2020.2.1844

Cited by / Share

Licence

This work is licensed under a Creative Commons Attribution 4.0 International License.

References

Bardetskiy A. B., Dębiec M., Potekhina I. D. and Saile T. 2017. Raptus Sabinae? Of a female calvarium from the Bandkeramik settlement of Rovantsi in Volhynia. Sprawozdania Archeologiczne 69, 235-251.
Google Scholar

Brandt G., Haak W., Adler C. J., Roth C., Szécsényi-Nagy A., Karimnia S., Möller-Rieker S., Meller H., Ganslmeier R., Friederich S., Dresely V., Nicklisch N., Pickrell J. K., Sirocko F., Reich D., Cooper A., Alt K. W. and Genographic Consortium. 2013. Ancient DNA reveals key stages in the formation of central European mitochondrial genetic diversity. Science. 2013 Oct 11; 342(6155), 257-261.
Google Scholar

Butler J. M. (ed.) 2015.Advanced Topics in Forensic DNA Typing: Interpretation. San Diego, CA, USA: Academic Press.
Google Scholar

Cerqueira C. C. S., Paixăo Côrtes V. R., Zambra F. M. B., Salzano F. M., Hünemeier T. and Bortolini M.C. 2012. Predicting homo pigmentation phenotype through genomic data: From neanderthal to James Watson. American Journal of Human Biology 24(5), 705-709.
Google Scholar

Chaitanya L., Breslin K., Zuńiga S., Wirken L., Pospiech E., Kukla-Bartoszek M., Sijen T., de Knijff P., Liu F., Branicki W., Kayser M. and Walsh S. 2018. The HIrisPlex-S system for eye, hair and skin colour prediction from DNA: Introduction and forensic developmental validation. Forensic Science International Genetics 35, 123-135.
Google Scholar

Dębiec M. and Saile T. 2015. Zu den östlichsten Siedlungen der frühen Bandkeramik. Praehistorische Zeitschrift 90, 1-19.
Google Scholar

Deng L. and Xu S. 2018. Adaptation of human skin color in various populations. Hereditas (2018) 155:1.
Google Scholar

Enattah N. S., Sahi T., Savilahti E., Terwilliger J. D., Peltonene L. and Järvelä I. 2002. Identification of a variant associated with adult-type hypolactasia. Nature Genetics 30, 233-237.
Google Scholar

Fehren-Schmitz L., Reindel M., Cagigao E. T., Hummel S. and Herrmann B. 2010. Pre-Columbian population dynamics in coastal southern Peru. A diachronic investigation of mtDNA pattern in the Palpa region by ancient DNA analysis. American Journal of Physical Anthropology 141(2), 208-221.
Google Scholar

Flux A. L., Mazanec J., Strommenger B. and Hummel S. 2017. Staphylococcus aureus Sequences from Osteomyelitic Specimens of a Pathological Bone Collection from Pre-Antibiotic Times. Diversity 9, 43.
Google Scholar

Frischalowski M., Seidenberg V., Grosskopf B., Wulf F. W. and Hummel S. 2015. Molekulargenetische Untersuchung des Verwandtschaftsverhältnisses von möglichen Mutter-Kind-Bestattungen aus dem frühneuzeitlichen Eldagsen. Nachrichten aus Niedersachsens Urgeschichte 84, 193-206.
Google Scholar

Fulge M. 2005. Laktosetoleranz in der bronzezeitlichen Lichtensteinhöhle – Molekulargenetischer Nachweis des Polymorphismus C/T 13910 an prähistorischer DNA. Göttingen 2005 [unpublished state examination thesis].
Google Scholar

Haak W., Forster P., Bramanti B., Matsumura S., Brandt G., Tänzer M., Villems R., Renfrew C., Gronenborn D., Alt K. W. and Burger J. 2005. Ancient DNA from the first European farmers in 7500-year-old Neolithic sites. Science 310(5750), 1016-1018.
Google Scholar

Haak W., Balanovsky O., Sanchez J. J., Koshel S., Zaporozhchenko V., Adler C. J., Der Sarkissian C. S. I., Brandt G., Schwarz C., Nicklisch N., Dresel V., Fritsch B., Balanovska E., Villems R., Meller H., Alt K. W., Cooper A. and the Genographic Consortium. 2010. Ancient DNA from European Early Neolithic Farmers Reveals Their Near Eastern Affinities. PLoS Biol. 2010 Nov 9;8(11): e1000536.
Google Scholar

Hofreiter M., Jaenicke V., Serre D., von Haeseler A. and Pääbo S. 2001. DNA sequences from multiple amplifications reveal artifacts induced by cytosine deamination in ancient DNA. Nucleic Acids Res. 29(23), 4793-4799.
Google Scholar

Hummel S. 2003. Ancient DNA Typing. Methods, Strategies, and Applications. Berlin, Heidelberg: Springer.
Google Scholar

Kayser M. 2015. Forensic DNA Phenotyping: Predicting human appearance from crime scene material for investigative purposes. Forensic Science International: Genetics 18, 33-48.
Google Scholar

Mathieson I., Lazaridis I., Rohland N., Mallick S., Patterson N., Roodenberg S., Harney E., Stewardson K., Fernandes D., Novak M., Sirak K., Gamba C., Jones E., Llamas B., Dryomov S., Pickrell J., Arsuaga J., Bermúdez de Castro J.-M., Carbonell E., Gerritsen F., Khokhlov A., Kuznetsov P., Lozano M., Meller H., Mochalov O., Moiseyev V., Guerra M. A. R., Roodenberg J., Vergčs J. M., Krause J., Cooper A., Alt K.W., Brown D., Anthony D., Lalueza-Fox C., Haak W., Pinhasi R. and Reich D. 2015. Genome-wide patterns of selection in 230 ancient Eurasians. Nature 528(7583), 499-503.
Google Scholar

Olalde I., Allentoft M. E., Sánchez-Quinto F., Santpere G., Chiang C. W. K., DeGiorgio M., Prado-Martínez J., Rodríguez J. A., Rasmussen S., Quilez J., Ramírez O., Marigorta U. M., Fernández-Callejo M., Prada M. E., Encinas J. M. V., Nielsen R., Netea M. G., Novembre J., Sturm R. A., Sabeti P., Marqučs-Bonet T., Navarro A., Willerslev E. and Lalueza-Fox C. 2014. Derived Immune and Ancestral Pigmentation Alleles in a 7,000-Year-old Mesolithic European. Nature 2014 March 13; 507(7491).
Google Scholar

Pala M., Olivieri A., Achilli A., Accetturo M., Metspalu E., Reidla M., Tamm E., Karmin M., Reisberg T., Hooshiar Kashani B., Perego U. A., Carossa V., Gandini F., Pereira J. B., Soares P., Angerhofer N., Rychkov S., Al-Zahery N., Carelli V., Sanati M. H., Houshmand M., Hatina J., Macaulay V., Pereira L., Woodward S. R., Davies W., Gamble C., Baird D., Semino O., Villems R., Torroni A. and Richards M. B. 2012. Mitochondrial DNA signals of late glacial recolonization of Europe from near eastern refugia. American Journal of Human Genetics 90(5), 915-924.
Google Scholar

Parson W. and Dür A. 2007. EMPOP – A forensic mtDNA database. Forensic Science International: Genetics 1(2), 88-92. https://empop.online/.
Google Scholar

Phillips C., Salas A., Sánchez J. J., Fondevila M., Gómez-Tato A., Álvarez-Dios J., Calaza M., Casares de Cal M., Ballard D., Lareu M. V., Carracedo Á. and The SNPforID Consortium. 2007. Inferring ancestral origin using a single multiplex assay of ancestry-informative marker SNPs. Forensic Science International: Genetics 1(3-4), 273-280. www.http://mathgene.usc.es/snipper/.
Google Scholar

Saile Th., Sedlmaier H. and Dębiec M. 2018. Šárka in Wolhynien und in Bayern. Ein Beitrag zu spätbandkeramischen Dreiecksintarsien. Archäologisches Korrespondenzblatt 48, 27-38.
Google Scholar

Schmidt N., Schücker K., Krause I., Dörk T., Klintschar M. and Hummel S. 2020. Genome-wide SNP typing of ancient DNA – Determination of hair and eye colour of Bronze Age humans from their skeletal remains. American Journal of Physical Anthropology 2020, 1-11.
Google Scholar

Seebode C. 2010. Das Skelettkollektiv der Lichtensteinhöhle als genetisches Archiv – Untersuchungen des Laktosetoleranzmarkers C/T13910 an den bronzezeitlichen Individuen. Göttingen 2010 [unpublished diploma thesis].
Google Scholar

Seidenberg V. 2016. Ein bronzezeitlicher Familienclan als genetisches Archiv – Morphologisch-paläogenetische Bearbeitung des Skelettkollektivs aus der Lichtensteinhöhle. Göttingen 2016 [unpublished PhD thesis].
Google Scholar

Seidenberg V., Schilz F., Pfister D., Georges L., Fehren-Schmitz L. and Hummel S. 2012. A new miniSTR heptaplex system for genetic fingerprinting of ancient DNA from archaeological human bone. Journal of Archaeological Science 39, 2012, 3224-3229.
Google Scholar

Söchtig J., Phillips C., Marońas O., Gómez-Tato A., Cruz R., Alvarez-Dios J., Casares de Cal M.-Á., Ruiz Y., Reich K., Fondevila M., Carracedo Á. and Lareu M. V. 2015. Exploration of SNP variants affecting hair colour prediction in Europeans. International Journal of Legal Medicine 129(5), 963-975.
Google Scholar

Strien H.-C. 2018.Westexpansion und Regionalisierung der Ältesten Bandkeramik (= Kommunikation und Wandel. Monographien zu Entwicklungen in der Urgeschichte 1). Kerpen-Loogh: Welt und Erde Verlag.
Google Scholar